Biol 2420, 006 Lab Schedule Fall 2024

UNIT 1 Cell and Colony Morphology

Week 1  

8-27.

                 Watch: 

                 Micro Lab Tutors: Safety in the Microbiology Laboratory

                 Micro Lab Explorations: Lab Safety

                 ACC Lab Safety Video

                 ACC Handgun policy Video

                 Read and Complete:

                 Media Lab Lab Notes in Blackboard

8-29

                 Watch:

                 Micro Lab Tutors: Aseptic Transfer of Bacteria

                 Micro Lab Explorations:  Aseptic Technique

                 Read and Complete:

                 Aseptic transfer Lab Notes in Blackboard

Week 2  

9-3

                 Watch:

                 Micro Lab Tutors: Streak Plate Technique

                 Micro Lab Explorations: Streak for Isolation

                 Catalyst U: Streaking for Isolation

9-5

                 Read and Complete:

                 Streak Plate Lab Notes in Blackboard

Week 3  

9-10

                 Watch:

                 Colony Morphology Power Point in Blackboard

                 Colony Samples from the Lab in Blackboard

                 Read and Complete:

                 Microbugz Lab Exercises

                               Microbial Growth

                                    Cultural Characteristics

                 Colony Morphology Lab Notes

9-12

                 Watch:

                 Micro Lab Tutor: Microscopy

                 Lab Technique Videos: 

                               Smear Preparation

                               Simple Staining

                               Negative Staining

                 Micro Lab Explorations:

                               Simple Stain

Power Point in Bb:  Preparation of Smears, Simple Stains, and Negative Stains

                 Read and complete as appropriate

                 Microbugz Lab Exercises

                               Microscopy and Staining

                                    Simple stains

                                    Negative stains

                 Microbugz Handouts

                               Microscope Use and Care Handout

                 Pearson Study Area

                               MicroLab Practical

                                    Parts of the Microscope

Week 4  

9-17

                 Watch:

                 Catalyst U:  Microbiology: Gram Staining

                 Micro Lab Explorations: Gram Stain

                 Power Point in Bb:

                 Catalyst U: Microbiology: Acid Fast, Endospore and Capsule Stain

                 Read:

                 Microbugz Lab Exercises

                               Microscopy and Stains

                                    Acid-Fast Stain

                                    Capsule Stain

                                    Endospore Stain

                               Differential Tests:

                                    Catalase Test

                 Microbugz Handouts

                               Microscope Use and Care Handout

                               Stain Chart Handout

                               Stain Protocols Handout

                               Catalase Handout

9-19

                 Watch:

                 Read and Complete:

                 Microbugz Handouts

                               Motility Handout

Week 5  

9-24. Morphological Unknown assignments

                 Morphological Data sheets and report in Blackboard

9-26. NO LAB  so that students can complete Unit 1 Exam and work on their Virtual Unknown #1, Morphological Unknown identification and data sheet.

Unit 2:  Identifying Gram negative Bacilli Organisms

Week 6. Selective and Differential Media for G- Organisms

10-1  & 10-3

Start with these two:

                               Identifying G- Bacilli PP in Blackboard

                               Pearson Study Area

                                    MicroLab Explorations

                                          Biochemical Tests for Gram-Negative Bacteria     

                 Watch:

                 Catalyst U videos

                               MAC Agar

                               EMB Agar

                               Triple Sugar Iron Agar

                 Videos in Bb

                               Phenol Red Broths

                 Read and Complete where applicable:

                 Microbugz Lab Exercises

                               Selective Media:

                                    MacConkey Agar (MAC)

                                    Eosin Methylene Blue Agar (EMB)                                                                                       Hektoen Enteric Agar (HE)

                               Differential  Tests:

                                    Phenol Red Broth

                                    Triple Sugar Iron/Kligler Iron Agar

                 Pearson Study Area

                               MicroLab Practical

                                    Biochemical Test Interpretation (TSI)

                                    Selective and Differential Media (Set 2) (EMB)

                 Identifying G- Bacteria Lab Notes JMS 2-26-24.docx

Week 7   Selective and Differential Media for G- Organisms

10-8 & 10-10

                 Watch:

                 Catalyst U videos

                               Indole test

                               Methyl Red test

                               Voges Proskauer test

                               Citrate Test

                 Videos in Bb

                               IMViC Panel. Indole, Methyl Red, Voges Proskauer, Citrate

                 Read and Complete where applicable:

                 Microbugz Lab Exercises

                               Differential Tests

                                     Methyl Red and Voges-Proskauer Tests

                                     Citrate Test

                                     SIM Medium

                                     Oxidase Test

                                     Decarboxylation Test

                 Pearson Study Area

                               MicroLab Practical

                                    Biochemical Tests (Set 4) (Indole)

                                    Biochemical Tests (Set 6) (Methyl Red)

                                    Biochemical Tests (Set 8) (VoguesProskauer)

                                    Biochemical Tests (Set 3) (Simmons Citrate)

                 Identifying G - Bacilli Lab Notes JMS 2-26-24.docx                                     

Week 8  

                 10/15 & 10/17

                 Watch:

                 Catalyst U videos:

                               Nitrate Reduction Test

                               Urea Hydrolysis

                               DNA Hydrolysis

                 ACC Videos:

                               Gelatin Hydrolysis

                 Pearson Study Area:

                               MicroLab Practical:

                                    Biochemical Tests (Set 1) (Urea Test)

                 Read and Complete where applicable:

                 Microbugz Lab Exercises:

                               Differential Tests

                                    Nitrate Reduction Test

                                    Urease Test

                                    DNase Test

                                    Urease Test

                                    Gelatinase Test

                                    Phenylalanine Deaminase Test

                 Identifying G - Bacilli Lab Notes JMS 2-26-24.docx

Week 9  

                 10/22

                               Classes will not meet so that students can take Unit 2 Exam

                 10/24

                               Assignment of Gram Negative Unknown, complete

Week 10

                 10/29 - HHMI Immunology Virtual Lab

                 10/31 – classes will work on Gram Negative Unknown Identification

______________________________________________________________________

Unit 3: Identifying Gram Positive Cocci Organisms

Week 11

                 11/5 & 11/7

                 Selective and Differential Media for G+ Organisms. In this unit we are learning about selective and differential media used to identify Gram-positive cocci bacteria. Please read and study the following related to these topics.

Blackboard
Identifying Gram+ Cocci Organisms.pptx
Identifying G+ Orgnaisms Lab Notes Sp 24 rev JMS edit (1).docx

                 Pearson’s Study Area
                               MicroLab Explorations

                                     Biochemical Tests for Gram Positive bacteria

                 WATCH

                               Catalyst U videos:

                                    5% Sheep Blood agar

                                    Bacitracin and Optochin Susceptibility (includes Novobiocin too)

                                    Mannitol Salt Agar

                                    Coagulase Test

                               ACC videos:

                                    Blood Agar Plates

                                    Bile Esculin Agar Test

                                    Kirby Bauer Antibiotic Sensitivity

                                    Mannitol Salt Agar                                                                

                                    Coagulase Test

                 READ and COMPLETE AS APPROPRIATE

                               MicroLab Explorations

                                    Biochemical Tests for Gram Positive organisms

                               Microbugz Lab Exercises

                                    Blood Agar

                                    Bile Esculin Test

                                    Bacitrcin & Optochin Susceptibility

                 Project #3 Gram Positive Unknown Identification
 

Week 12

                 11/12 & 11/14

                 WATCH

                 READ AND COMPLETE

11/6                        Antimicrobial Susceptibility Test (Kirby-Bauer Method)

                               Chemical Germicides: Disinfectants and Antiseptics        

                                Anaerobic Jar                                                                           

                               Effects of UV Radiation                                                          

                               Assign Gram + unknown project                                           

                 11/8                                                                                                                       Continue Gram + unknown project     

Week 13 11/12    no lab scheduled, work online on your gram + unknown project  

                 11/14    The Fungi—Common Yeasts and Molds                            

                                                                   Continue gram + unknown project           

Week 14 11/19            catch up if necessary                                                     

                 11/21            no lab is scheduled so that students can complete the                                                   online Semester Exam

Week 15 11/26    Ex. 12-2,3,4 Helminth Parasites, Nematode Parasites, Hookworms                                            p.801 - 813                                              

                 11/28    Ex. 12-4 Helminth Parasites, Nematode Parasites, Hookworms  p.813 

Week 16 12/3                                                Gram+ Unknown project is Due

                                                         Optional oral presentation of Gram + idenification

                 12/5                                                                                              No Lab

Biol 2420, 006 Lab Schedule Fall 2024

UNIT 1 Cell and Colony Morphology

Week 1  

8-27.

                 Watch: 

                 Micro Lab Tutors: Safety in the Microbiology Laboratory

                 Micro Lab Explorations: Lab Safety

                 ACC Lab Safety Video

                 ACC Handgun policy Video

                 Read and Complete:

                 Media Lab Lab Notes in Blackboard

8-29

                 Watch:

                 Micro Lab Tutors: Aseptic Transfer of Bacteria

                 Micro Lab Explorations:  Aseptic Technique

                 Read and Complete:

                 Aseptic transfer Lab Notes in Blackboard

Week 2  

9-3

                 Watch:

                 Micro Lab Tutors: Streak Plate Technique

                 Micro Lab Explorations: Streak for Isolation

                 Catalyst U: Streaking for Isolation

9-5

                 Read and Complete:

                 Streak Plate Lab Notes in Blackboard

Week 3  

9-10

                 Watch:

                 Colony Morphology Power Point in Blackboard

                 Colony Samples from the Lab in Blackboard

                 Read and Complete:

                 Microbugz Lab Exercises

                               Microbial Growth

                                    Cultural Characteristics

                 Colony Morphology Lab Notes

9-12

                 Watch:

                 Micro Lab Tutor: Microscopy

                 Lab Technique Videos: 

                               Smear Preparation

                               Simple Staining

                               Negative Staining

                 Micro Lab Explorations:

                               Simple Stain

Power Point in Bb:  Preparation of Smears, Simple Stains, and Negative Stains

                 Read and complete as appropriate

                 Microbugz Lab Exercises

                               Microscopy and Staining

                                    Simple stains

                                    Negative stains

                 Microbugz Handouts

                               Microscope Use and Care Handout

                 Pearson Study Area

                               MicroLab Practical

                                    Parts of the Microscope

Week 4  

9-17

                 Watch:

                 Catalyst U:  Microbiology: Gram Staining

                 Micro Lab Explorations: Gram Stain

                 Power Point in Bb:

                 Catalyst U: Microbiology: Acid Fast, Endospore and Capsule Stain

                 Read:

                 Microbugz Lab Exercises

                               Microscopy and Stains

                                    Acid-Fast Stain

                                    Capsule Stain

                                    Endospore Stain

                               Differential Tests:

                                    Catalase Test

                 Microbugz Handouts

                               Microscope Use and Care Handout

                               Stain Chart Handout

                               Stain Protocols Handout

                               Catalase Handout

9-19

                 Watch:

                 Read and Complete:

                 Microbugz Handouts

                               Motility Handout

Week 5  

9-24. Morphological Unknown assignments

                 Morphological Data sheets and report in Blackboard

9-26. NO LAB  so that students can complete Unit 1 Exam and work on their Virtual Unknown #1, Morphological Unknown identification and data sheet.

Unit 2:  Identifying Gram negative Bacilli Organisms

Week 6. Selective and Differential Media for G- Organisms

10-1  & 10-3

Start with these two:

                               Identifying G- Bacilli PP in Blackboard

                               Pearson Study Area

                                    MicroLab Explorations

                                          Biochemical Tests for Gram-Negative Bacteria     

                 Watch:

                 Catalyst U videos

                               MAC Agar

                               EMB Agar

                               Triple Sugar Iron Agar

                 Videos in Bb

                               Phenol Red Broths

                 Read and Complete where applicable:

                 Microbugz Lab Exercises

                               Selective Media:

                                    MacConkey Agar (MAC)

                                    Eosin Methylene Blue Agar (EMB)                                                                                       Hektoen Enteric Agar (HE)

                               Differential  Tests:

                                    Phenol Red Broth

                                    Triple Sugar Iron/Kligler Iron Agar

                 Pearson Study Area

                               MicroLab Practical

                                    Biochemical Test Interpretation (TSI)

                                    Selective and Differential Media (Set 2) (EMB)

                 Identifying G- Bacteria Lab Notes JMS 2-26-24.docx

Week 7   Selective and Differential Media for G- Organisms

10-8 & 10-10

                 Watch:

                 Catalyst U videos

                               Indole test

                               Methyl Red test

                               Voges Proskauer test

                               Citrate Test

                 Videos in Bb

                               IMViC Panel. Indole, Methyl Red, Voges Proskauer, Citrate

                 Read and Complete where applicable:

                 Microbugz Lab Exercises

                               Differential Tests

                                     Methyl Red and Voges-Proskauer Tests

                                     Citrate Test

                                     SIM Medium

                                     Oxidase Test

                                     Decarboxylation Test

                 Pearson Study Area

                               MicroLab Practical

                                    Biochemical Tests (Set 4) (Indole)

                                    Biochemical Tests (Set 6) (Methyl Red)

                                    Biochemical Tests (Set 8) (VoguesProskauer)

                                    Biochemical Tests (Set 3) (Simmons Citrate)

                 Identifying G - Bacilli Lab Notes JMS 2-26-24.docx                                     

Week 8  

                 10/15 & 10/17

                 Watch:

                 Catalyst U videos:

                               Nitrate Reduction Test

                               Urea Hydrolysis

                               DNA Hydrolysis

                 ACC Videos:

                               Gelatin Hydrolysis

                 Pearson Study Area:

                               MicroLab Practical:

                                    Biochemical Tests (Set 1) (Urea Test)

                 Read and Complete where applicable:

                 Microbugz Lab Exercises:

                               Differential Tests

                                    Nitrate Reduction Test

                                    Urease Test

                                    DNase Test

                                    Urease Test

                                    Gelatinase Test

                                    Phenylalanine Deaminase Test

                 Identifying G - Bacilli Lab Notes JMS 2-26-24.docx

Week 9  

                 10/22

                               Classes will not meet so that students can take Unit 2 Exam

                 10/24

                               Assignment of Gram Negative Unknown, complete

Week 10

                 10/29 - HHMI Immunology Virtual Lab

                 10/31 – classes will work on Gram Negative Unknown Identification

______________________________________________________________________

Unit 3: Identifying Gram Positive Cocci Organisms

Week 11

                 11/5 & 11/7

                 Selective and Differential Media for G+ Organisms. In this unit we are learning about selective and differential media used to identify Gram-positive cocci bacteria. Please read and study the following related to these topics.

Blackboard
Identifying Gram+ Cocci Organisms.pptx
Identifying G+ Orgnaisms Lab Notes Sp 24 rev JMS edit (1).docx

                 Pearson’s Study Area
                               MicroLab Explorations

                                     Biochemical Tests for Gram Positive bacteria

                 WATCH

                               Catalyst U videos:

                                    5% Sheep Blood agar

                                    Bacitracin and Optochin Susceptibility (includes Novobiocin too)

                                    Mannitol Salt Agar

                                    Coagulase Test

                               ACC videos:

                                    Blood Agar Plates

                                    Bile Esculin Agar Test

                                    Kirby Bauer Antibiotic Sensitivity

                                    Mannitol Salt Agar                                                                

                                    Coagulase Test

                 READ and COMPLETE AS APPROPRIATE

                               MicroLab Explorations

                                    Biochemical Tests for Gram Positive organisms

                               Microbugz Lab Exercises

                                    Blood Agar

                                    Bile Esculin Test

                                    Bacitrcin & Optochin Susceptibility

                 Project #3 Gram Positive Unknown Identification
 

Week 12

                 11/12 & 11/14

                 WATCH

                 READ AND COMPLETE

11/6                        Antimicrobial Susceptibility Test (Kirby-Bauer Method)

                               Chemical Germicides: Disinfectants and Antiseptics        

                                Anaerobic Jar                                                                           

                               Effects of UV Radiation                                                          

                               Assign Gram + unknown project                                           

                 11/8                                                                                                                       Continue Gram + unknown project     

Week 13 11/12    no lab scheduled, work online on your gram + unknown project  

                 11/14    The Fungi—Common Yeasts and Molds                            

                                                                   Continue gram + unknown project           

Week 14 11/19            catch up if necessary                                                     

                 11/21            no lab is scheduled so that students can complete the                                                   online Semester Exam

Week 15 11/26    Ex. 12-2,3,4 Helminth Parasites, Nematode Parasites, Hookworms                                            p.801 - 813                                              

                 11/28    Ex. 12-4 Helminth Parasites, Nematode Parasites, Hookworms  p.813 

Week 16 12/3                                                Gram+ Unknown project is Due

                                                         Optional oral presentation of Gram + idenification

                 12/5                                                                                              No Lab

An introduction to the microbial world including the basic characteristics of fungi, algae, bacteria, and viruses. Special emphasis is placed on applications to humans

Student Learning Outcomes/Learning Objectives

Course-Level: Specific skills/competencies are expected of students who successfully complete this course, including the ability to:

• Explain microbiological processes in detail and on an appropriate level, including cell theory, metabolism, genetics, control, and interactions between humans and microbes, including the disease process and defense/immune responses
• Correctly perform microbiologic lab skills such as use of a Bunsen burner, use of the microscope, aseptic technique, streaking for isolation, establishing pure cultures, and isolation and determination of unknowns using differential stains and differential/selective media
• Display a habit of safe lab practices including aseptic technique and disposal of biohazard waste

Department objectives

• Discuss the germ theory of disease and its development.
• Discuss aseptic procedures for the preparation of media and materials for the culturing and       growth of microbes.
• Enumerate and differentiate among the different groups of organisms included for study in microbiology.
• Discuss and distinguish between eukaryotic and prokaryotic cell types.
• Discuss energy acquisition and utilization by microbes and the function of enzymes in cellular activities.
• Discuss microbial metabolism, including anabolic pathways and glycolytic, fermentative and respiratory catabolic pathways.
• Discuss fundamental nucleic acid chemistry regarding the principle of complementarity, DNA replication, the genetic code, protein synthesis, metabolic regulation, and cellular reproduction.
• Discuss microbial genetics including, but not restricted to sexual versus asexual means of reproduction, and transformation, transduction and conjugation in bacteria.
• Discuss methods utilized in biotechnology as applied to microbes, including the role of microbes and biotechnology in industrial microbiology, the pharmaceutical and food industries, and systematic and diagnostic microbiology.
• Discuss symbiotic relationships, including commensal, mutualistic, and parasitic relationships among hosts and microbes.
• Discuss disease processes, the cellular structures, metabolic and genetic activities, and biological and chemical agents employed by microbes in colonizing, infecting, invading, and causing diseases in hosts.
• Discuss the processes, both nonspecific and specific, employed by hosts in resisting the onslaught of infectious diseases.
• Discuss the principles and methods of diagnosing diseases, identifying disease-causing agents, and tracking and enumerating diseases around the world.
• Discuss the signs, symptoms, etiology, course, prevention, control, diagnosis, and treatment for the most common infectious diseases of all organ systems of the human.

• General Education- As a Core Curriculum course, students completing this course will demonstrate competence in:

• Critical Thinking – gathering, analyzing, synthesizing, evaluating, and applying information.
• Interpersonal Skills – interacting collaboratively to achieve common goals.
• Quantitative and Empirical Reasoning – Applying mathematical, logical, and scientific principles and methods.
• Written/Oral/Visual Communication – Communicating effectively, adapting to purpose, structure, audience, & medium.