Spring 2024

Micro Lab Explorations (MLE) in the MyLab and Mastering Study Area (prelab exercises)

Microbugz (MBZ) link in Blackboard  Please print the Microbugz lab exercise and/or Handout (check both areas) and bring it to lab during the scheduled week.                    

Week 1   1/17       Ubiquity of Microorganisms..... ……………Microbugz Lab Exercises

                              Comparison of Hand-Cleaning Agents …..Microbugz, Hand Scrubbing

Week 2   1/22       Lab Familiarization & Safety Guidelines, ACC video

                               Lab Safety … Laboratory Rules, MBZ handout

                 1/24       Aseptic Technique …………..Aseptic Technique, MLE and MBZ (fundamental skills)

                                    Flaming a loop………………..MBZ handout

                                  Inoculation ……………………MBZ Handout

Begin data on Virtual Unknown #1 Handout (Blackboard link in Week 2)

                               Streak Plate Method of Isolation… Streak for Isolation, MLE and MBZ (fundamental skills)

Week 3   1/29       Colony Morphology….…Cultural Characteristics, MBZ

                 1/31       Introduction to the Light Microscope…

                                     Online Study area, Lab Technique videos, Compound Microscope.

                                      MBZ, Microscope Use and Care Handout

                               Microscopic Examination of Freshwater Microbes may be available.

Week 4   2/5         Simple Stains. MLE prelab activity, MBZ, Stain Protocols

                               Negative Stains..MLE prelab activity, MBZ Stain Protocols

                 2/7         Gram Stain….MLE prelab activity, MBZ, Stain Protocols

                               Begin Morphological unknown…….

                               ................................. using Morphological UNK #1 in Bb.

Week 5   2/12       Gram Stain Morphological of Morphological Unknown , see Week 4

                               Capsule Stain…………..Mastering, video structural stains
                                     ……………………….MBZ Stain Protocols, MBZ Stain Protocols

                 2/14       Acid-Fast Stain…….MLE prelab activity, MBZ Stain Protocols

                               Endospore Stain. Mastering, video, structural stains; MLE prelab activity

                               ....................................... Continue Morphological Unknown

Week 6   2/19       ............ no lab so that students can take online Exam I

                               Ex. 5-28 ............................................... Motility Test Medium........... p. 437

                               Ex. 5-6.............................................................. Catalase Test........... p. 321

                                ....................................... Continue Morphological Unknown

                 2/21       Motility Test Medium……………….…MBZ Handout and Lab exercise

                               Catalase Test…………………..……...MBZ Handout and Lab exercise

Finish Morphological Unknown

Week 7   2/26       Selective and Differential Media for G- Organisms.

• MLE video for Gram negative bacteria activity
• Complete the Lab Notes, Unit 2. Selective & Differential media for G(-) Bacilli
• Complete Unknown #2: Making a Flowchart

Eosin Methylene Blue Agar (EMB).............................................................. MBZ

Hektoen Enteric Agar (HE)........................................................................... MBZ

MacConkey Agar (MAC).................................................................. MLE & MBZ

Phenol Red Broth.............................................................................. MLE & MBA

Triple Sugar Iron/Kligler Iron Agar............................................................... MBZ

                 2/28       .............................................. Read results from last session

                               ............................................ Morphological Unknown Due

Week 8   3/4         Methyl Red and Voges-Proskauer Tests....... MLE & MBZ

                               Citrate Test....................................................................... MBZ

                               SIM Medium........................................................ MLE & MBZ

                               Oxidase Test....................................................... MLE & MBZ

                               Decarboxylation Test...................................................... MBZ

                 3/6         .............................................. Read results from last session

SPRING BREAK – NO CLASS

Week 9   3/18       Nitrate Reduction Test................................................... MBZ

                               Urea Hydrolysis (Broth).................................................. MBZ

                               DNA Hydrolysis Test...................................................... MBZ

                               Gelatin Hydrolysis Test.................................................. MBZ

                               Phenylalanine Deaminase Test.................................... MBZ

                 3/20       .............................................. Read results from last session

Week 10 3/25       .......... No Lab will meet so that students can take Exam II

                 3/27       ....................................................................... College Closed

Week 11 4/2         Selective and Differential Media for G+ Organisms

• View MLE video for Gram (+) organisms
•  

                               Columbia CNA with 5% Sheep Blood Agar

                               Bile Esculin Test.............................................................. MBZ

                               6.5% Salt Broth............................................................... MBZ

                               Bacitracin & Optochin Antibiotic Susceptibility Tests MBZ

                               Novobiocin Antibiotic Susceptibility Test use Above protocol

                               Begin Gram negative and positive unknowns

                               Use the

                               Unknown #2, Making a G(-) Flowchart and

                               Unknown #3, Gram (-) Gram (+) Dichotomous Key/Flowchart)

Week 11 4/4         Blood Agar and Novobiocin susceptibility...... MLE & MBZ

                               Mannitol Salt Agar (MSA)................................. MLE & MBZ

                               Coagulase Test............................................................... MBZ

                                GRAM (–) Unknown due

Week 12 4/8        Antimicrobial Susceptibility Test (Kirby-Bauer Method) see above for antibiotic tests….also MLE

                               Chemical Germicides: Disinfectants and Antiseptics........

• Control of Microbial Growth…MLE

                               Anaerobic Jar................................................................... MBZ

                               Effects of UV Radiation..................................................... tbd

                               Continue Gram (+) Unknown Unknown...............................

                 4/10       Read results from last session

                               Continue Gram (+) Unknown.................................................

Week 13 4/15       Lab will not meet so that students can take Exam III

                 4/17       The Fungi—Common Yeasts and Mold………tbd

                               ................................................ Continue Gram (+) Unknown...........

Week 14 4/22       Examination of Common Protozoan……..tbd

                               ................................................ Continue Gram (+) Unknown

                 4/24       Examination of Common Protozoans ............................ tbd

                               ........................................... Continue Gram (+) if necessary

                 5/1         Helminth Parasites, Nematode Parasites, Hookworms …..tbd

Week 16 5/6         ........................................................ Gram (+) Unknown due

                 5/8         ...................................................................................... No Lab

The E-Textbook 

• Microbiology With Diseases By Body System 6th editions 
  Author:  Robert Bauman

is included in your tuition.  You may opt out of this and obtain a print copy through the Blackboard ink.

The Lab Manual will be in Blackboard as individual exercises and virtual labs that are part of your online resources for the Textbook.

Spring 2024

Micro Lab Explorations (MLE) in the MyLab and Mastering Study Area (prelab exercises)

Microbugz (MBZ) link in Blackboard  Please print the Microbugz lab exercise and/or Handout (check both areas) and bring it to lab during the scheduled week.                    

Week 1   1/17       Ubiquity of Microorganisms..... ……………Microbugz Lab Exercises

                              Comparison of Hand-Cleaning Agents …..Microbugz, Hand Scrubbing

Week 2   1/22       Lab Familiarization & Safety Guidelines, ACC video

                               Lab Safety … Laboratory Rules, MBZ handout

                 1/24       Aseptic Technique …………..Aseptic Technique, MLE and MBZ (fundamental skills)

                                    Flaming a loop………………..MBZ handout

                                  Inoculation ……………………MBZ Handout

Begin data on Virtual Unknown #1 Handout (Blackboard link in Week 2)

                               Streak Plate Method of Isolation… Streak for Isolation, MLE and MBZ (fundamental skills)

Week 3   1/29       Colony Morphology….…Cultural Characteristics, MBZ

                 1/31       Introduction to the Light Microscope…

                                     Online Study area, Lab Technique videos, Compound Microscope.

                                      MBZ, Microscope Use and Care Handout

                               Microscopic Examination of Freshwater Microbes may be available.

Week 4   2/5         Simple Stains. MLE prelab activity, MBZ, Stain Protocols

                               Negative Stains..MLE prelab activity, MBZ Stain Protocols

                 2/7         Gram Stain….MLE prelab activity, MBZ, Stain Protocols

                               Begin Morphological unknown…….

                               ................................. using Morphological UNK #1 in Bb.

Week 5   2/12       Gram Stain Morphological of Morphological Unknown , see Week 4

                               Capsule Stain…………..Mastering, video structural stains
                                     ……………………….MBZ Stain Protocols, MBZ Stain Protocols

                 2/14       Acid-Fast Stain…….MLE prelab activity, MBZ Stain Protocols

                               Endospore Stain. Mastering, video, structural stains; MLE prelab activity

                               ....................................... Continue Morphological Unknown

Week 6   2/19       ............ no lab so that students can take online Exam I

                               Ex. 5-28 ............................................... Motility Test Medium........... p. 437

                               Ex. 5-6.............................................................. Catalase Test........... p. 321

                                ....................................... Continue Morphological Unknown

                 2/21       Motility Test Medium……………….…MBZ Handout and Lab exercise

                               Catalase Test…………………..……...MBZ Handout and Lab exercise

Finish Morphological Unknown

Week 7   2/26       Selective and Differential Media for G- Organisms.

• MLE video for Gram negative bacteria activity
• Complete the Lab Notes, Unit 2. Selective & Differential media for G(-) Bacilli
• Complete Unknown #2: Making a Flowchart

Eosin Methylene Blue Agar (EMB).............................................................. MBZ

Hektoen Enteric Agar (HE)........................................................................... MBZ

MacConkey Agar (MAC).................................................................. MLE & MBZ

Phenol Red Broth.............................................................................. MLE & MBA

Triple Sugar Iron/Kligler Iron Agar............................................................... MBZ

                 2/28       .............................................. Read results from last session

                               ............................................ Morphological Unknown Due

Week 8   3/4         Methyl Red and Voges-Proskauer Tests....... MLE & MBZ

                               Citrate Test....................................................................... MBZ

                               SIM Medium........................................................ MLE & MBZ

                               Oxidase Test....................................................... MLE & MBZ

                               Decarboxylation Test...................................................... MBZ

                 3/6         .............................................. Read results from last session

SPRING BREAK – NO CLASS

Week 9   3/18       Nitrate Reduction Test................................................... MBZ

                               Urea Hydrolysis (Broth).................................................. MBZ

                               DNA Hydrolysis Test...................................................... MBZ

                               Gelatin Hydrolysis Test.................................................. MBZ

                               Phenylalanine Deaminase Test.................................... MBZ

                 3/20       .............................................. Read results from last session

Week 10 3/25       .......... No Lab will meet so that students can take Exam II

                 3/27       ....................................................................... College Closed

Week 11 4/2         Selective and Differential Media for G+ Organisms

• View MLE video for Gram (+) organisms
•  

                               Columbia CNA with 5% Sheep Blood Agar

                               Bile Esculin Test.............................................................. MBZ

                               6.5% Salt Broth............................................................... MBZ

                               Bacitracin & Optochin Antibiotic Susceptibility Tests MBZ

                               Novobiocin Antibiotic Susceptibility Test use Above protocol

                               Begin Gram negative and positive unknowns

                               Use the

                               Unknown #2, Making a G(-) Flowchart and

                               Unknown #3, Gram (-) Gram (+) Dichotomous Key/Flowchart)

Week 11 4/4         Blood Agar and Novobiocin susceptibility...... MLE & MBZ

                               Mannitol Salt Agar (MSA)................................. MLE & MBZ

                               Coagulase Test............................................................... MBZ

                                GRAM (–) Unknown due

Week 12 4/8        Antimicrobial Susceptibility Test (Kirby-Bauer Method) see above for antibiotic tests….also MLE

                               Chemical Germicides: Disinfectants and Antiseptics........

• Control of Microbial Growth…MLE

                               Anaerobic Jar................................................................... MBZ

                               Effects of UV Radiation..................................................... tbd

                               Continue Gram (+) Unknown Unknown...............................

                 4/10       Read results from last session

                               Continue Gram (+) Unknown.................................................

Week 13 4/15       Lab will not meet so that students can take Exam III

                 4/17       The Fungi—Common Yeasts and Mold………tbd

                               ................................................ Continue Gram (+) Unknown...........

Week 14 4/22       Examination of Common Protozoan……..tbd

                               ................................................ Continue Gram (+) Unknown

                 4/24       Examination of Common Protozoans ............................ tbd

                               ........................................... Continue Gram (+) if necessary

                 5/1         Helminth Parasites, Nematode Parasites, Hookworms …..tbd

Week 16 5/6         ........................................................ Gram (+) Unknown due

Course-Level: Specific skills/competencies are expected of students who successfully complete this course, including the ability to:

• Explain microbiological processes in detail and on an appropriate level, including cell theory, metabolism, genetics, control, and interactions between humans and microbes, including the disease process and defense/immune responses
• Correctly perform microbiologic lab skills such as use of a Bunsen burner, use of the microscope, aseptic technique, streaking for isolation, establishing pure cultures, and isolation and determination of unknowns using differential stains and differential/selective media
• Display a habit of safe lab practices including aseptic technique and disposal of biohazard waste

Department objectives

• Discuss the germ theory of disease and its development.
• Discuss aseptic procedures for the preparation of media and materials for the culturing and       growth of microbes.
• Enumerate and differentiate among the different groups of organisms included for study in microbiology.
• Discuss and distinguish between eukaryotic and prokaryotic cell types.
• Discuss energy acquisition and utilization by microbes and the function of enzymes in cellular activities.
• Discuss microbial metabolism, including anabolic pathways and glycolytic, fermentative and respiratory catabolic pathways.
• Discuss fundamental nucleic acid chemistry regarding the principle of complementarity, DNA replication, the genetic code, protein synthesis, metabolic regulation, and cellular reproduction.
• Discuss microbial genetics including, but not restricted to sexual versus asexual means of reproduction, and transformation, transduction and conjugation in bacteria.
• Discuss methods utilized in biotechnology as applied to microbes, including the role of microbes and biotechnology in industrial microbiology, the pharmaceutical and food industries, and systematic and diagnostic microbiology.
• Discuss symbiotic relationships, including commensal, mutualistic, and parasitic relationships among hosts and microbes.
• Discuss disease processes, the cellular structures, metabolic and genetic activities, and biological and chemical agents employed by microbes in colonizing, infecting, invading, and causing diseases in hosts.
• Discuss the processes, both nonspecific and specific, employed by hosts in resisting the onslaught of infectious diseases.
• Discuss the principles and methods of diagnosing diseases, identifying disease-causing agents, and tracking and enumerating diseases around the world.
• Discuss the signs, symptoms, etiology, course, prevention, control, diagnosis, and treatment for the most common infectious diseases of all organ systems of the human.

• General Education- As a Core Curriculum course, students completing this course will demonstrate competence in:

• Critical Thinking – gathering, analyzing, synthesizing, evaluating, and applying information.
• Interpersonal Skills – interacting collaboratively to achieve common goals.
• Quantitative and Empirical Reasoning – Applying mathematical, logical, and scientific principles and methods.
• Written/Oral/Visual Communication – Communicating effectively, adapting to purpose, structure, audience, & medium.